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Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment

Identifieur interne : 000126 ( Main/Exploration ); précédent : 000125; suivant : 000127

Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment

Auteurs : Sung-Hwan Choi [Corée du Sud] ; Won-Seok Jeong [Corée du Sud] ; Jung-Yul Cha [Corée du Sud] ; Jae-Hoon Lee [Corée du Sud] ; Kee-Joon Lee [Corée du Sud] ; Hyung-Seog Yu [Corée du Sud] ; Eun-Ha Choi [Corée du Sud] ; Kwang-Mahn Kim [Corée du Sud] ; Chung-Ju Hwang [Corée du Sud]

Source :

RBID : PMC:5476562

Abstract

We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for 15 min and then tested them immediately and after storage for 28 days, with and without distilled H2O (dH2O). We evaluated the surface characteristics using surface profiling, contact angle analysis, X-ray photoelectron spectroscopy, and in terms of the surface zeta-potential. We determined the level of biological activity by analysing albumin adsorption, MC3T3-E1 and human mesenchymal cell adhesion and cytoskeleton development, as well as the production of intracellular reactive oxygen species between groups. The surface characteristics produced by the UV irradiation were maintained in dH2O for 28 days. We found that titanium stored in dH2O for 28 days after UV treatment exhibited enhanced protein adsorption, cell attachment, and cytoskeleton development. Titanium stored in dH2O for 28 days after UV irradiation exhibited a lower level of oxidative stress, comparable to that of the titanium immediately after UV treatment. UV treatment combined with wet storage can be used as a means of overcoming the biological aging of titanium.


Url:
DOI: 10.1038/s41598-017-04192-9
PubMed: 28630441
PubMed Central: 5476562


Affiliations:


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<p>We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for 15 min and then tested them immediately and after storage for 28 days, with and without distilled H
<sub>2</sub>
O (dH
<sub>2</sub>
O). We evaluated the surface characteristics using surface profiling, contact angle analysis, X-ray photoelectron spectroscopy, and in terms of the surface zeta-potential. We determined the level of biological activity by analysing albumin adsorption, MC3T3-E1 and human mesenchymal cell adhesion and cytoskeleton development, as well as the production of intracellular reactive oxygen species between groups. The surface characteristics produced by the UV irradiation were maintained in dH
<sub>2</sub>
O for 28 days. We found that titanium stored in dH
<sub>2</sub>
O for 28 days after UV treatment exhibited enhanced protein adsorption, cell attachment, and cytoskeleton development. Titanium stored in dH
<sub>2</sub>
O for 28 days after UV irradiation exhibited a lower level of oxidative stress, comparable to that of the titanium immediately after UV treatment. UV treatment combined with wet storage can be used as a means of overcoming the biological aging of titanium.</p>
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<name sortKey="Hwang, Chung Ju" sort="Hwang, Chung Ju" uniqKey="Hwang C" first="Chung-Ju" last="Hwang">Chung-Ju Hwang</name>
<name sortKey="Jeong, Won Seok" sort="Jeong, Won Seok" uniqKey="Jeong W" first="Won-Seok" last="Jeong">Won-Seok Jeong</name>
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